RBS

Part:BBa_K4212019

Designed by: Youzi Bi   Group: iGEM22_Imperial_College_London   (2022-09-30)
Revision as of 18:51, 13 October 2022 by Helen737 (Talk | contribs)


Optimal_RBS

Very strong RBS in B. subtilis, increases expression to 25 folds.

Usage and Biology

Ribosome binds to this place and start translation. Optimal RBS can improve the efficiency.

References

[1] Nowroozi, F.F., Baidoo, E.E.K., Ermakov, S., Redding-Johanson, A.M., Batth, T.S., Petzold, C.J. & Keasling, J.D. (2014) Metabolic pathway optimization using ribosome binding site variants and combinatorial gene assembly. Applied Microbiology and Biotechnology. 98 (4), 1567–1581. doi:10.1007/s00253-013-5361-4.

[2] McLaughlin, J.R., Murray, C.L. & Rabinowitz, J.C. (1981) Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene. Journal of Biological Chemistry. 256 (21), 11283–11291. doi:10.1016/S0021-9258(19)68589-3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None