Coding

Part:BBa_K4212010

Designed by: Youzi Bi   Group: iGEM22_Imperial_College_London   (2022-09-30)
Revision as of 16:07, 30 September 2022 by Registry (Talk | contribs)

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GerAK_modified_chimera

Synthetic operon designed with GerAA, GerKB and GerAC coding sequences from the B. subtilis genome. Designed to be transformed in a B. subtilis knockout strain so as to produce a germinant receptor that contains a binding site for glucose, and when activated triggers an orthogonal signalling cascade resulting in germination. It is an improved version of the GerAK chimera.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 992
    Illegal EcoRI site found at 1308
    Illegal PstI site found at 943
    Illegal PstI site found at 2762
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 992
    Illegal EcoRI site found at 1308
    Illegal PstI site found at 943
    Illegal PstI site found at 2762
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 992
    Illegal EcoRI site found at 1308
    Illegal BglII site found at 524
    Illegal XhoI site found at 2869
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 992
    Illegal EcoRI site found at 1308
    Illegal PstI site found at 943
    Illegal PstI site found at 2762
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 992
    Illegal EcoRI site found at 1308
    Illegal PstI site found at 943
    Illegal PstI site found at 2762
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 179


[edit]
Categories
Parameters
None