Coding

Part:BBa_K4212009

Designed by: Shirin Bamezai   Group: iGEM22_Imperial_College_London   (2022-09-29)
Revision as of 21:43, 29 September 2022 by Sbamezai (Talk | contribs)


GerAK_chimera

Synthetic operon designed with GerAA, GerKB and GerAC coding sequences from the B. subtilis genome. Designed to be transformed in a B. subtilis knockout strain so as to produce a germinant receptor that contains a binding site for glucose, and when activated triggers an orthogonal signalling cascade resulting in germination. The GerAA sequence features a C-terminus fusion to a GFPmut3b coding sequence. This is to enable visualisation of the chimeric receptor on the surface of the spore via fluorescence microscopy.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2384
    Illegal EcoRI site found at 4339
    Illegal PstI site found at 533
    Illegal PstI site found at 4290
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2384
    Illegal EcoRI site found at 4339
    Illegal NheI site found at 4
    Illegal PstI site found at 533
    Illegal PstI site found at 4290
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2384
    Illegal EcoRI site found at 4339
    Illegal BglII site found at 3871
    Illegal XhoI site found at 640
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2384
    Illegal EcoRI site found at 4339
    Illegal PstI site found at 533
    Illegal PstI site found at 4290
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2384
    Illegal EcoRI site found at 4339
    Illegal PstI site found at 533
    Illegal PstI site found at 4290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3526


[edit]
Categories
Parameters
None