Coding

Part:BBa_K4212008:Design

Designed by: Shirin Bamezai   Group: iGEM22_Imperial_College_London   (2022-09-29)
Revision as of 16:22, 29 September 2022 by Registry (Talk | contribs)

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GerA_operon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3506
    Illegal PstI site found at 521
    Illegal PstI site found at 3457
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3506
    Illegal PstI site found at 521
    Illegal PstI site found at 3457
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3506
    Illegal BglII site found at 3038
    Illegal XhoI site found at 628
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3506
    Illegal PstI site found at 521
    Illegal PstI site found at 3457
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3506
    Illegal PstI site found at 521
    Illegal PstI site found at 3457
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2693


Design Notes

Codon optimised to remove recognition sites for BbsI, BsaI and BsmbI to enable assembly in Golden Gate system.


Source

Native sequence from B. subtilis genome.

References