Coding

Part:BBa_K4212007

Designed by: Shirin Bamezai   Group: iGEM22_Imperial_College_London   (2022-09-29)
Revision as of 16:17, 29 September 2022 by Registry (Talk | contribs)

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Cas9

Codon optimised Cas9 CDS for B. subtilis. Useful for CRISPR-Cas9 experiments in B. subtilis. Used in the Sporadicate project to develop a self-digesting plasmid system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3823
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3823
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3823
    Illegal BglII site found at 1188
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3823
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3823
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None