![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4212007
Designed by: Shirin Bamezai Group: iGEM22_Imperial_College_London (2022-09-29)
Cas9
Codon optimised Cas9 CDS for B. subtilis. Useful for CRISPR-Cas9 experiments in B. subtilis. Used in the Sporadicate project to develop a self-digesting plasmid system.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3823
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3823
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3823
Illegal BglII site found at 1188 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3823
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3823
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |