Part:BBa_K4307025

AffiPmrB

This part is modified from PmrB receptor in PmrB/A two-component system in E.coli. PmrB uses an iron (III)-binding motif to sense the environment(Chen & Groisman, 2013). The extracellular iron (III)-binding motif of pmrB receptor is replaced with another protein named affibody to construct this part. Affibody is a protein that can recognize Fc fragment of antibody(Stahl et al., 2017). This part is used to recognize the extracellular antibody signal and start the downstream signal transduction system.
In Tsinghua 2022 project, it was used in affipmrABC（BBa_K4307028) system.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 855
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

The following figure demonstrates our successful construction.

Figure 1: The construction results of AffiPmrB.

References

[1] Chen, H. D., & Groisman, E. A. (2013). The biology of the PmrA/PmrB two-component system: the major regulator of lipopolysaccharide modifications. Annu Rev Microbiol, 67, 83-112.

[2] Stahl, S., Graslund, T., Eriksson Karlstrom, A., Frejd, F. Y., Nygren, P. A., & Lofblom, J. (2017). Affibody Molecules in Biotechnological and Medical Applications. Trends Biotechnol, 35(8), 691-712.