Coding

Part:BBa_K4165154

Designed by: Salma Sobhy Sadek   Group: iGEM22_CU_Egypt   (2022-09-30)
Revision as of 17:21, 10 October 2022 by M Zaki (Talk | contribs)


MM3

Tau binding peptide targeting the PHF seed of Tau

Usage and Biology

The amino acid sequence of the MM3 peptide is DPLKARHTSVWY. It is the L-enantiomeric peptide of MMD3 (BBa_K4165141). Its charge is +1, hydrophobicity is 41 %, molecular weight is 1472.65 Da. MM3 can reduce aggregates of full-length tau when mixed in ratio 1:10 and 1:5 and PHF* (VQIINK) but not PHF (VQIVYK).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Dry Lab

Modeling

MM3 is modeled by Apptest, ITASSER and TrRosetta, best model obtained from trrosetta. 



                            Figure 1.: Predicted 3D structure of Synthetic peptide MM3.


Table 1: Quality assessment parameters of MM3 model.


References

1. Malhis, M. (2021). Selection and characterization of D-enantiomeric peptides for the investigation of options for therapy and diagnosis of Alzheimer's disease . University of Bayreuth (Germany).


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