Coding

Part:BBa_K4307026

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-09-30)
Revision as of 15:53, 12 October 2022 by YuheWang (Talk | contribs)


PmrA

This part is the transcription factor of Affi-PmrB/A system（BBa_K4307028）, which is modified from the PmrB/A two-component system in E.coli. It is phosphorylated by AffipmrB, then it can activate PmrC promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Characterization

The following figure demonstrates our successful construction.


bba-k4307026-gel.png

               Figure 1:  The construction results of PmrA.


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Categories
Parameters
None