Reporter
Part:BBa_K228862
Designed by: Haoqian Zhang Group: iGEM09_PKU_Beijing (2009-09-22)
salP-GFP
T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 408
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1725
[edit]
Categories
Parameters
n/a | salP-GFP |