Coding

Part:BBa_K4307012

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-09-30)
Revision as of 15:47, 12 October 2022 by Junyao-z20 (Talk | contribs)


NisC

Originated from Lactococcus lactis subsp. Lactis, NisC is involved in the post-translational modification of the lantibiotic nisin. We managed to express NisC in E.coli for post-translational modification of NisA-affi fusion protein.
Functions together with NisA-affi(BBa_K4307010) and NisB(BBa_K4307011), it forms composite part NisA-affi-NisB+NisC (BBa_K4307014) to produce functional NisA-affibody peptide.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 541
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 541
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 541
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 541
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

The following figure demonstrates our successful construction.


bba-k4307012-gel.png

               Figure 1:  The construction results of NisC.


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Categories
Parameters
None