Regulatory

Part:BBa_K4307009

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-09-30)
Revision as of 15:43, 12 October 2022 by Junyao-z20 (Talk | contribs)


No part name specified with partinfo tag.

Originated from Lactococcus lactis subsp. Lactis, PnisA is a member of the two-component regulatory system NisK/NisR involved in the regulation of the biosynthesis of lantibiotic nisin. PnisA functions as a promoter which is activated by phosphorylated NisR and enhance downstream gene expression in response to environmental nisin signals. We managed to involve PnisA sequence in plasmids for E.coli strain.
Functions together with NisK(BBa_K4307007), NisR(BBa_K4307008), J23100 and EGFP, it forms composite part J23100-nisK-nisR+PnisA-EGFP(BBa_K4307013) to express fluorescence signal induced by nisin.


Sequence and Features No part name specified with partinfo tag.


Characterization

The following figure demonstrates our successful construction.


bba-k4307009-gel.png

               Figure 1:  The construction results of PnisA.


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