Composite

Part:BBa_K4260006

Designed by: Claudia Angélica García Alonso   Group: iGEM22_TecCEM   (2022-10-12)
Revision as of 15:21, 12 October 2022 by Registry (Talk | contribs)

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Isoeugenol Monooxygenase, inducible coding sequence: Trp term, IemR, promoter, pelB, Iso & rrnBT1 te

BBa_K4260006 is a composite part that aims to work as a selection marker for the replacement of antibiotic-based selection markers. Isoeugenol monooxygenase (coded by gene Iso) is an enzyme present in Pseudomonas bacteria, that converts isoeugenol to vanillin directly. Its transcription is regulated by the expression of its transcriptional activator (IemR), as well as the presence of isoeugenol.


Isoeugenol is an aromatic compound found in clover and cinnamon essential oils, that has proved to have an inhibitory effect on different microorganisms; however, this genetic construct was designed for its expression in Escherichia coli. Its antimicrobial activity resides in altering the integrity of the inner membrane of the bacteria, leading to fluid spillage and cell death. Therefore, by directing the enzyme to the periplasm of the bacteria, only transformed cells could survive when exposed to isoeugenol.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 439
    Illegal XhoI site found at 267
    Illegal XhoI site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1187
    Illegal NgoMIV site found at 1895
    Illegal NgoMIV site found at 2503
    Illegal AgeI site found at 1719
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1123
    Illegal BsaI.rc site found at 1815


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