Measurement

Part:BBa_K4511007

Designed by: Wenhe Ma   Group: iGEM22_HUS_United   (2022-10-12)
Revision as of 14:15, 12 October 2022 by Registry (Talk | contribs)

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Reporter device with J23106 , B0032, and GFP for dtRNA1 strength measurement

This part is a measurement device for degradation-tuning RNAs, which contains medium strength J23106 promoter, medium RBS B0032, and a reporter protein coding sequence GFP. The effectiveness of dtRNA is evaluated by measuring GFP fluorescence and bacterial growth curve. It could be used as an inter-lab study device to compare experimental conditions among different labs. This plasmid could also be directly transformed into other species or strains of E.coli to study the effectiveness of dtRNA on another chassis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 753


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