Composite
PK

Part:BBa_K4324003:Design

Designed by: Chris Yoo   Group: iGEM22_TheKingsSchool_AU_HS   (2022-10-02)
Revision as of 13:33, 2 October 2022 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


lacPO-RBS-PK-T1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 210
    Illegal BamHI site found at 507
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1912
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protein sequence was codon-optimised for Escherichia coli through the IDT codon optimisation tool as gBlocks Gene Fragments.



Source

XFP gene from Bifidobacterium animalis subsp. lactis (strain DSM 10140 / JCM 10602 / LMG 18314)

References