Plasmid

Part:BBa_K4361115

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-08)
Revision as of 08:49, 11 October 2022 by Larsvdb (Talk | contribs)


pSB1C3 with GHB / GBL reporter

This composite part shows the GHB / GBL reporter system designed by the Bielefeld-CeBiTec iGEM 2015 team (Part:BBa_K1758376) in the pSB1C3 plasmid. As such, this part shows the linearized sequence of both parts after digestion with EcoRI and PstI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2029
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2029
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2029
    Illegal XhoI site found at 1013
    Illegal XhoI site found at 1905
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2029
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2029
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2156


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Categories
Parameters
None