Device
Part:BBa_K176240
Designed by: Chao Li, Hao Jiang Group: iGEM09_USTC (2009-10-14)
High input score automatic selector without lethal device: PoPS->luxI-LVA->K176237->PoPS
PoPS->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS Output PoPS = (Cell Density * (pCon 0.36 + Population Average Input PoPS) + Input AHL) / (1 + Input PoPS)
If output is a lethal device, cells with relative high input score in the population will be selected, cells with relative low input score will die.
The first luxI-LVA is used to calculate average score in the population.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1524
Illegal NheI site found at 1547
Illegal NheI site found at 2373
Illegal NheI site found at 2396 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 655
Illegal BglII site found at 2215 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3351
[edit]
Categories
Parameters
n/a | High input score automatic selector without lethal device: PoPS->luxI-LVA->K176237->PoPS |