Composite

Part:BBa_K4273021

Designed by: Su Junzhe   Group: iGEM22_LINKS_China   (2022-09-30)
Revision as of 12:28, 30 September 2022 by Registry (Talk | contribs)

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pTDH3-Np5598-tTDH1-pPGK1-Np5597-tPGK1-pTEF2-NlmysH-tSSA1

Gene from cyanobacteria N. punctiforme and Nostoc linkia NIES-25 involved in biosynthesis of shinorine.

encoding ATP-grasp ligase(AG-L) that converts 4-deoxygadusol (4-DG) to mycosporine-glycine(MG), D-Ala-D-Ala ligase(ALA-L) that converts mycosporine-glycine(MG) to Shinorine and 2-oxoglutarate-dependent oxygenase (MysH）that converts shinorine or porphyra-334 to palythine


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3423
    Illegal BglII site found at 5516
    Illegal XhoI site found at 2075
    Illegal XhoI site found at 5936
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2321
    Illegal BsaI site found at 5922
    Illegal BsaI.rc site found at 3433
    Illegal BsaI.rc site found at 6172
    Illegal SapI.rc site found at 5741
    Illegal SapI.rc site found at 5862


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