Part:BBa_K4275013

OlpB-3C-Ag3

OlpB3CAg3, an outer layer protein B with 3 type II cohesins and an antigen 3 (Ag3), is the secondary scaffoldin of cellulosome complex. The main function of OlpB is securing cellulosome integrating protein A (CipA) to the cell surface. The type II cohesin interacts with the type II dockerin on CipA, binding CipA to OlpB. In turn, OlpB adheres to the cell surface through the interaction between Ag3 and nanobody 3 (Nb3) present on the cell surface. The binding to the cell surface effectively increases the efficiency of the cellulosome complex. This is a part in a part collection where we enable efficient degradation of cellulose and PET in textile waste.

Figure 1 The 3D structure of the protein predicted by Alphafold2.

Usage and Biology

OlpB3CAg3 is the secondary scaffoldin of cellulosome complex that has 3 type II cohesins and an antigen 3 (Ag3). Natural OlpB is found in Clostridium thermocellum, a gram-positive thermophilic and anaerobic bacterium. OlpB is responsible for binding several primary scaffoldin of cellulosome complex, CipA, to the cell surface [1]. OlpB binds to CipA via the interaction between the type II dockerin and type II cohesin. In nature, OlpB attaches to the cell surface of Clostridium thermocellum through the interaction between surface-like homologous (SLH) domains and the cell surface. Together, the CipA1B2C, OlpB, and the cellulosomal enzymes comprise a multiplex cellulosome. Utilizing the highly effective cellulosome complex, C.thermocellum is the most efficient microorganism for lignocellulosic biomass degradation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 706
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1252
  Illegal NgoMIV site found at 1341
  Illegal AgeI site found at 183
  Illegal AgeI site found at 319
  Illegal AgeI site found at 370
  Illegal AgeI site found at 502
  Illegal AgeI site found at 868
  Illegal AgeI site found at 886
  Illegal AgeI site found at 1312
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1069
  Illegal SapI.rc site found at 1081
  Illegal SapI.rc site found at 1705
  

References

1. Anandharaj, Marimuthu et al. "Constructing A Yeast To Express The Largest Cellulosome Complex On The Cell Surface". Proceedings Of The National Academy Of Sciences, vol 117, no. 5, 2020, pp. 2385-2394. Proceedings Of The National Academy Of Sciences, https://doi.org/10.1073/pnas.1916529117.