Primer

Part:BBa_K4361112

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-08)
Revision as of 08:46, 11 October 2022 by Larsvdb (Talk | contribs)


Blc operator SDM primer F

A site-directed mutagenesis primer, to be used in conjunction with the reverse primer Part:BBa_K4361113. When used as primers during the PCR of pSB1C3 containing the Blc operator sequence as an insert (Part:BBa_K4361115), a deletion will be induced in the operator, resulting in the altered sequence as described in Part:BBa_K4361114. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence may act as a negative control when testing the binding properties of BlcR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None