Part:BBa_K4477014
Anti-mouse camelid VHH - complete expression cassette
This sequence codes for a heavy chain variable fragment (VHH), also known as a nanobody, that binds to the Fc region of murine IgGs. The sequence contains a promoter, RBS, gene sequence of nanobody, and terminator. Like the other composite parts designed by Virginia 2022, this part is expressed by IPTG induction. First, a strong T7 promoter occurs on the 5' end of the DNA sequence, facilitating IPTG induction. Then, an RBS the team optimized using the Salis RBS calculator for expression of the nanobody coding sequence occurs. The coding sequence for the nanobody occurs six base pairs downstream of the 3' end of the RBS, as recommended by [choose one of many sources] for optimal ribosomal translation. Lastly, a two-way terminator completes the composite part; as a two-way terminator, it not only prevents RNA polymerases from reading past the composite part into the backbone, but prevents any genes in the backbone from being transcribed into the composite part and interfering with their expression. Note that between each subcomponent, legal restriction enzyme sites were included for modularity.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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