Composite

Part:BBa_K4238006

Designed by: Keita Hoshino   Group: iGEM22_UTokyo   (2022-10-09)
Revision as of 10:42, 12 October 2022 by Shundroid (Talk | contribs)


ADH1p+Cre+CYC1t

Cre is an enzyme that performs specific sequence recombination and can be used to delete portions of sequences irreversibly.

Cre has the enzymatic activity of circularly excising the region flanked by recognition sequences called loxP. This part was created to assay the recombination . Since we intended to use it in yeast, the homeostatic promoters ADH1 and CYC1 terminator are those that function in yeast.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 919
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None