DNA

Part:BBa_K4162029

Designed by: Weiwen Chen   Group: iGEM22_Fudan   (2022-10-06)
Revision as of 07:51, 7 October 2022 by Ted Chen (Talk | contribs)


ribozyme + T7_RBS + BCMO + ribozyme + T7_RBS + ybbO-tdMCP-EGFP

Introduction

2021 Fudan

Coding sequence of BCMO and gene ybbO separated by hammerhead ribozyme. Gene ybbO is tagged with tdMCP and EGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2521
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2521
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2521
    Illegal AgeI site found at 1018
    Illegal AgeI site found at 1611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2101
    Illegal BsaI site found at 2455


[edit]
Categories
Parameters
None