Composite

Part:BBa_K4204019:Design

Designed by: Jianfei Song   Group: iGEM22_BNDS_China   (2022-10-11)
Revision as of 15:13, 11 October 2022 by Jianfei Song (Talk | contribs) (→‎References)


IPTG induced GFP improved with ProQC system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 316
    Illegal NheI site found at 1116
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 978


Design Notes

The GFP could be replaced by any protein in interest.


Source

The protein quality control system is from Yang et al's work [1]. Other components are from BBa_K653003.Assembled by BNDS_China 2022.

References

[1] Yang, J., Han, Y.H., Im, J. et al. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol 17, 421–427 (2021). https://doi.org/10.1038/s41589-021-00736-3