Plasmid_Backbone

Part:BBa_K4501022:Design

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-22)
Revision as of 13:30, 22 September 2022 by Registry (Talk | contribs)

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pJUMP lentiviral transfer plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 5061
    Illegal suffix found at 5105
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5061
    Illegal SpeI site found at 5106
    Illegal PstI site found at 5120
    Illegal NotI site found at 5067
    Illegal NotI site found at 5113
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5061
    Illegal BglII site found at 33
    Illegal BglII site found at 74
    Illegal BglII site found at 3682
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5061
    Illegal suffix found at 5106
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5061
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5076
    Illegal SpeI site found at 5106
    Illegal PstI site found at 5120
    Illegal NgoMIV site found at 1895
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2693
    Illegal SapI.rc site found at 4194


Design Notes

We had to remove the BsmBI recognition sites of the MSCV modules.


Source

Designed by ourselves.

References