Part:BBa_K3036005:Experience
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Applications of BBa_K3036005
As for the killing switch, we refer to the arabinos-induced suicide system of 2019-BNU_China, which uses arabinos-induced promoter (pBAD) to express the toxic protein MazF. So when the patient takes in arabinos, the engineering bacteria will commit a suicide. When we repeat the experimental methods provided by 2019-NEU_China, we found that the results are not good. We repeat 5 times and no expected outcome was achieved. As we thought maybe we failed to build the part, our advisor Xiaoting Luan reminded us that our bacteria grow very fast——2h to achieve the logarithmic phase, 4h to reached the plateau phase, 6h to recession phase.
We also got in touch with 2019-BNU_China and discussed the method. So we shorten the experiment time and after we did that, the outcome proved that our part was effective.
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