Protein_Domain
Part:BBa_K4181020
Designed by: Junzhi Yan Group: iGEM22_SJTU-BioX-Shanghai (2022-10-05)
cotE→His tag→linker
CotE as an anchor protein can be used to bind melanin to spores. To test whether it is expressed, a His tag is used for verification, followed by a linker to link the subsequent cotB protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 298
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 298
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 303
Illegal XhoI site found at 491 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 298
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 298
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1571
[edit]
Categories
Parameters
None |