Signalling
Part:BBa_K266000
Designed by: UNAM FCIENCIAS-CINVESTAV IRA Group: iGEM09_IPN-UNAM-Mexico (2009-10-13)
PAI+LasR -> LuxI (AI)
This biobrick has a promoter inducible by PAI+LasR (BBa_R0079)and produces LuxI enzyme (BBa_F1610). This enzyme produces 3OC6HSL (AI).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 58
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 820
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//direction/forward
//function/cellsignalling/LasR
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//function/cellsignalling/LasR
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
biology | |
control | PAI |
direction | Forward |
negative_regulators | |
o_h | |
o_l | |
positive_regulators | 1 |