RNA

Part:BBa_K4169010

Designed by: Jie Min   Group: iGEM22_HZAU-China   (2022-09-30)
Revision as of 10:24, 12 October 2022 by Felicitysm (Talk | contribs) (Usage and Biology)


A RNA thermometer mutation 1

Based on the characteristics of secondary structure changes of RNA under different temperature conditions, the RNA thermometer element was introduced in this project. An RC sequence is present on this RNA thermometer, which recruits RNase E to degrade subsequent RNA fragments. At low temperatures,such as 27℃, RNA thermometers will maintain the secondary stem-loop structure, and RC sequences will complement and pair with other bases, unable to recruit RNase, so subsequent sequences will be expressed normally. However, at 37℃, the secondary structure opens, and the subsequent RNA fragments are cleaved and degraded by RNA enzymes.We want to change the temperature at which it functions by mutating its gene sequence.So we mutated its gene sequence, that is, the 8th A in front of ARC was changed to G.

Design and Mutation

We designed the mutation primers (Figure 1.)<b>and the expected mutation sequences<2>(Figure 2.)<2></p> 无标题文档

Figure 1.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 39


[edit]
Categories
Parameters
None