Composite

Part:BBa_K4201018

Designed by: David Birkhaeuser   Group: iGEM22_CU-Boulder   (2022-10-12)
Revision as of 08:04, 12 October 2022 by Registry (Talk | contribs)

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CrtE_cytoTDS2-MBP_RUBY

This construct is split up into three main gene segments. At the center of each segment lies a gene of interest, in this case CrtE, cytoTDS2-MBP, and RUBY, respectively. Each segment is flanked upstream by the promoter Gmubi and downstream by the terminator AtHSP. See design tab for more information on gene origin.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4087
    Illegal PstI site found at 2087
    Illegal PstI site found at 3774
    Illegal PstI site found at 5335
    Illegal PstI site found at 6893
    Illegal PstI site found at 12101
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2087
    Illegal PstI site found at 3774
    Illegal PstI site found at 5335
    Illegal PstI site found at 6893
    Illegal PstI site found at 12101
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4087
    Illegal PstI site found at 2087
    Illegal PstI site found at 3774
    Illegal PstI site found at 5335
    Illegal PstI site found at 6893
    Illegal PstI site found at 12101
    Illegal NgoMIV site found at 1697
    Illegal NgoMIV site found at 8249
    Illegal NgoMIV site found at 8828
    Illegal NgoMIV site found at 10541
    Illegal AgeI site found at 3923
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 948
    Illegal BsaI site found at 1887
    Illegal BsaI site found at 2167
    Illegal BsaI site found at 3114
    Illegal BsaI site found at 6693
    Illegal BsaI site found at 6973
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 1873
    Illegal BsaI.rc site found at 2153
    Illegal BsaI.rc site found at 3100
    Illegal BsaI.rc site found at 6679
    Illegal BsaI.rc site found at 6959
    Illegal BsaI.rc site found at 7906


[edit]
Categories
Parameters
None