![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4201018
Designed by: David Birkhaeuser Group: iGEM22_CU-Boulder (2022-10-12)
CrtE_cytoTDS2-MBP_RUBY
This construct is split up into three main gene segments. At the center of each segment lies a gene of interest, in this case CrtE, cytoTDS2-MBP, and RUBY, respectively. Each segment is flanked upstream by the promoter Gmubi and downstream by the terminator AtHSP. See design tab for more information on gene origin.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4087
Illegal PstI site found at 2087
Illegal PstI site found at 3774
Illegal PstI site found at 5335
Illegal PstI site found at 6893
Illegal PstI site found at 12101 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2087
Illegal PstI site found at 3774
Illegal PstI site found at 5335
Illegal PstI site found at 6893
Illegal PstI site found at 12101 - 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4087
Illegal PstI site found at 2087
Illegal PstI site found at 3774
Illegal PstI site found at 5335
Illegal PstI site found at 6893
Illegal PstI site found at 12101
Illegal NgoMIV site found at 1697
Illegal NgoMIV site found at 8249
Illegal NgoMIV site found at 8828
Illegal NgoMIV site found at 10541
Illegal AgeI site found at 3923 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 948
Illegal BsaI site found at 1887
Illegal BsaI site found at 2167
Illegal BsaI site found at 3114
Illegal BsaI site found at 6693
Illegal BsaI site found at 6973
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 1873
Illegal BsaI.rc site found at 2153
Illegal BsaI.rc site found at 3100
Illegal BsaI.rc site found at 6679
Illegal BsaI.rc site found at 6959
Illegal BsaI.rc site found at 7906
[edit]
Categories
Parameters
None |