Protein_Domain

Part:BBa_K4181025

Designed by: Junzhi Yan   Group: iGEM22_SJTU-BioX-Shanghai   (2022-10-11)
Revision as of 11:52, 11 October 2022 by Registry (Talk | contribs)

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Dsup- K198M_V195G

We directed the evolution of the DSUP protein to change lysine at position 198 to methionine and valine at position 195 to glutamic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 163
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 121
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 197


[edit]
Categories
Parameters
None