Composite

Part:BBa_K4201020

Designed by: David Birkhaeuser   Group: iGEM22_CU-Boulder   (2022-10-12)
Revision as of 08:23, 12 October 2022 by Registry (Talk | contribs)

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CrtE_cytoTDS2__T5αOH_RUBY

This construct is made of four separate segments: CrtE, cytoTDS2, T5αOH, and RUBY. Each segment is enclosed by an upstream Gmubi promoter and downstream AtHSP terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6848
    Illegal PstI site found at 2087
    Illegal PstI site found at 5638
    Illegal PstI site found at 8395
    Illegal PstI site found at 13603
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6848
    Illegal PstI site found at 2087
    Illegal PstI site found at 5638
    Illegal PstI site found at 8395
    Illegal PstI site found at 13603
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6848
    Illegal BglII site found at 790
    Illegal BglII site found at 1153
    Illegal BglII site found at 2021
    Illegal BglII site found at 2956
    Illegal BglII site found at 4011
    Illegal BglII site found at 5324
    Illegal BglII site found at 5572
    Illegal BamHI site found at 11815
    Illegal XhoI site found at 3601
    Illegal XhoI site found at 3868
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6848
    Illegal PstI site found at 2087
    Illegal PstI site found at 5638
    Illegal PstI site found at 8395
    Illegal PstI site found at 13603
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6848
    Illegal PstI site found at 2087
    Illegal PstI site found at 5638
    Illegal PstI site found at 8395
    Illegal PstI site found at 13603
    Illegal NgoMIV site found at 1697
    Illegal NgoMIV site found at 6997
    Illegal NgoMIV site found at 9751
    Illegal NgoMIV site found at 10330
    Illegal NgoMIV site found at 12043
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 948
    Illegal BsaI site found at 1887
    Illegal BsaI site found at 2167
    Illegal BsaI site found at 3113
    Illegal BsaI site found at 5438
    Illegal BsaI site found at 5718
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 1873
    Illegal BsaI.rc site found at 2153
    Illegal BsaI.rc site found at 3100
    Illegal BsaI.rc site found at 5424
    Illegal BsaI.rc site found at 5704
    Illegal BsaI.rc site found at 6651


[edit]
Categories
Parameters
None