Composite
Part:BBa_K4236102
Designed by: Hanpeng Chen Group: iGEM22_iBowu-China (2022-10-12)
T7-RBS-atF3H-atFLS+atUGT
The whole pathway in plants to produce astragalin contains three enzymes, F3H, FLS, and UGT. We want to construct a big plasmid consisting of three genes we have introduced to produce astragalin. In order to better express each enzyme, we tried to connect the smaller F3H and FLS with a linker to form a fusion protein, and the larger UGT was expressed separately. Therefore, AtF3H-AtFLS + AtUGT expression vector was constructed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |