RBS

Part:BBa_K4477006:Design

Designed by: Miranda Khoury   Group: iGEM22_Virginia   (2022-09-06)
Revision as of 02:04, 7 September 2022 by Mirandakhoury (Talk | contribs) (→‎Design Notes)


RBS for expression of human/mouse anti-apoB(MDA) antibody light chain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used a pre-existing RBS, BBa_J61100, as a starting point for Salis optimization. We aimed for 75% relative expression so as to get a high level of protein expression of BBa_K4477001 without overburdening the E. coli expressing the gene.

We adjusted the RBS sequence to remove restriction sites that would make the RBS incompatible with various assembly methods.

Source

https://salislab.net/software/

References