Part:BBa_K4155002

pzntR+ ribB+ OprF

pzntR+ ribB Promoter sequence with recognition site for ZntR transcriptional regulator protein. ZntR activates transcription when Zn(II) is bound (1). ribB;the 3,4 dihydroxy-2-butanone-4-phosphate synthase; We get this part from E.coli genome by synthetize. It can increase the electric output by synthesis of riboflavin. Besides, riboflavin itself is yellow. After the addition of ribB, the bacteria will be yellow. The Microbial Fuel Cell (MFC) can be a future environmentally friendly biotechnological application for the production of electrical energy. As a future alternative energy source, the bioelectricity generation must become more efficient. A major limiting factor is the low bacterial membrane permeability, hindering transport of electron shuttles through the membrane and thereby restricting the electron shuttle-mediated extracellular electron transfer (EET) from bacteria to electrodes. This results in a reduced electrical power output of the MFC. Therefore, we heterologously expressed the porin protein OprF from Pseudomonas fluorescens into Escherichia coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 504
  Illegal BglII site found at 1561
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1953
  Illegal AgeI site found at 394
  Illegal AgeI site found at 436
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1474