Coding
Part:BBa_K4477004:Design
Designed by: Miranda Khoury Group: iGEM22_Virginia (2022-09-06)
McPC603 anti-oxLDL scFv antibody fragment
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 355
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 661
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Amino acid sequences reverse transcribed and codon optimized for expression in E. coli B (parent strain of SHuffle). Illegal restriction sites removed via codon optimization.
For detailed annotations at to which nucleotides form the variable light, variable heavy, and linker sequence domains, see Team Virginia 2022's Benchling folder here: https://benchling.com/mrkhoury/f_/YR4zZSUb-virginia-igem-2022-dna-constructs/
Source
1. https://www.sciencedirect.com/science/article/pii/S0769262585800581
2. https://pubmed.ncbi.nlm.nih.gov/14644097/