Composite

Part:BBa_K4444004

Designed by: Adiva Daniar, Shreya Vallimanalan   Group: iGEM22_UPenn   (2022-10-10)
Revision as of 04:44, 12 October 2022 by Ciperez (Talk | contribs) (→‎Sequence and Features)


PhoCl Display GFP

PhoCl Display mRuby Backbone is modified from the RELEASE system. It contains a GFP as the protein of interest, PhoCl2c, a tri-transmembrane domain and an ER retention motif. The furin cut site and HCV cut site were removed. PhoCl Display will display a protein of interest on the cell membrane in response to ultraviolet light stimulation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 898
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1021
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 60
    Illegal BsaI.rc site found at 1543


[edit]
Categories
Parameters
None