Coding

Part:BBa_K4477004:Design

Designed by: Miranda Khoury   Group: iGEM22_Virginia   (2022-09-06)
Revision as of 07:57, 12 October 2022 by Mirandakhoury (Talk | contribs) (→‎Source)


McPC603 anti-oxLDL scFv antibody fragment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 355
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 661
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequences reverse transcribed and codon optimized for expression in E. coli B (parent strain of SHuffle).


Source

1. https://www.sciencedirect.com/science/article/pii/S0769262585800581 - sequence of IK17
2. https://pubmed.ncbi.nlm.nih.gov/14644097/ - (G4S)3 sequence

References