Composite

Part:BBa_K4444002

Designed by: Cristina Perez, Adiva Daniar, June Ahn, Wangari Mbuthia, Shreya Vallimanalan   Group: iGEM22_UPenn   (2022-10-10)
Revision as of 07:50, 12 October 2022 by Ciperez (Talk | contribs)

PhoCl Secrete GFP

PhoCl Secrete mRuby is modified from the RELEASE system. It contains a GFP as a protein of interest, a furin cut site, PhoCl2c, tri-transmembrane domain, and an ER retention motif. PhoCl Secrete will completely release a protein of interest in response to ultra violet light stimulation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 946
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 60
    Illegal BsaI.rc site found at 1468


[edit]
Categories
Parameters
None