Composite

Part:BBa_K4229047:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-29)
Revision as of 18:43, 29 September 2022 by Registry (Talk | contribs)

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minimal wiffleball with tags under regulation of lambdapLhybrid promoter and LacI promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 444
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 444
    Illegal NotI site found at 1515
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 444
    Illegal BglII site found at 453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 444
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 444
    Illegal NgoMIV site found at 335
    Illegal NgoMIV site found at 842
    Illegal AgeI site found at 1319
    Illegal AgeI site found at 1427
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

Was kindly send to us by the Kerfeld group. From this paper https://doi.org/10.1073/pnas.2116871119

References