![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4201016:Design
Designed by: David Birkhaeuser Group: iGEM22_CU-Boulder (2022-10-12)
Crte-cytoTDS-MBP_RUBY
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1002
Illegal PstI site found at 4800
Illegal PstI site found at 5648
Illegal PstI site found at 10856 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1002
Illegal PstI site found at 4800
Illegal PstI site found at 5648
Illegal PstI site found at 10856 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 790
Illegal BglII site found at 1618
Illegal BglII site found at 3156
Illegal BglII site found at 4203
Illegal BglII site found at 5582
Illegal BglII site found at 6517
Illegal BglII site found at 10419
Illegal BamHI site found at 5254
Illegal BamHI site found at 9068
Illegal XhoI site found at 2579 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1002
Illegal PstI site found at 4800
Illegal PstI site found at 5648
Illegal PstI site found at 10856 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1002
Illegal PstI site found at 4800
Illegal PstI site found at 5648
Illegal PstI site found at 10856
Illegal NgoMIV site found at 7004
Illegal NgoMIV site found at 7583
Illegal NgoMIV site found at 9296
Illegal AgeI site found at 2321 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 948
Illegal BsaI site found at 5448
Illegal BsaI site found at 5728
Illegal BsaI site found at 6675
Illegal BsaI site found at 10656
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 5434
Illegal BsaI.rc site found at 5714
Illegal BsaI.rc site found at 6661
Illegal BsaI.rc site found at 10642
Illegal BsaI.rc site found at 10922
Illegal SapI.rc site found at 3377
Illegal SapI.rc site found at 3583
Design Notes
Part information and design consideration can be found on respective parts pages.
Source
Crte-cytoTDS-MBP is a de novo synthesized construct using genes from Pantoea ananatis LMG 20103, Taxus brevifolia. Synthesis was completed by iGem sponsors IDT and Twist Biosciences.