Part:BBa_K4385025
PbrR location detection
This component is made up of TurboID and PbrR. TurboID, a pair of biotin ligases, was designed to overcome the problem of slow existing biotin ligases.PbrR is a lead ion adsorption protein.
Usage and Biology
The PbrR without absorbing lead ions was connected and formed a complex under TurbolD's traction. With the combination of the biotin and streptavdin-cy3, the fluorescent signal can be observed. So we can detect the quantity of the monomer pbrR protein which can combine with the turboid-pbrR fusion protein.
Characterization
Our surface display system can express the Lpp-OmpA-pbrR protein in the outer membrane.So we use the turboid-pbrR to test the pbrR function.With the expression of Lpp-OmpA-pbrR, our bacteria have the ability to adsorb the Pb2+.
Our purpose is to detect the quantity of the monomer pbrR protein which can combine with the turboid-pbrR fusion protein.And with the combination of the biotin and streptavdin-cy3, the fluorescent signal can be observed.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1486
Illegal PstI site found at 1428 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 1486
Illegal PstI site found at 1428 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1486
Illegal PstI site found at 1428 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1486
Illegal PstI site found at 1428 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1219
None |