Coding

Part:BBa_K4488000

Designed by: Jessica Zhang   Group: iGEM22_Sydney_Australia   (2022-09-30)
Revision as of 05:54, 30 September 2022 by Registry (Talk | contribs)

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Neae: N terminus of 𝛃-intimin for Surface Display in E. coli

Intimin is an adhesion protein of EHEC. The N-terminus of beta-intimin (Neae) has been used for surface display in E. coli, specifically for sdAbs/Nanobodies. Neae consists of a trans-membrane beta barrel and requires the BAM complex for activation.

Reference: https://doi.org/10.1111%2F1751-7915.12819

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None