Coding
Part:BBa_K4477001:Design
Designed by: Miranda Khoury Group: iGEM22_Virginia (2022-09-06)
Human/mouse anti-apoB(MDA) (anti-oxLDL) IgG antibody - light chain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 382
Design Notes
Amino acid sequence was reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle).
Source
Anti-apoB(MDA) light chain amino acid sequence acquired from IMGT: https://www.imgt.org/3Dstructure-DB/cgi/details.cgi?pdbcode=9635