Plasmid

Part:BBa_K4182011

Designed by: Dan Tan   Group: iGEM22_XJTU-China   (2022-10-10)
Revision as of 03:15, 11 October 2022 by Registry (Talk | contribs)

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Temperature regulated suicide circuit

In addition to population-responsive suicide mechanisms, suicide systems with other regulatory modes can also be designed through synthetic biology. Here, we designed a temperature-responsive cleavage system to achieve temperature-controlled cleavage, that is, cleavage of thermoregulated lysis genes (Gene ID: IF654_RS00240)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1018
    Illegal suffix found in sequence at 1446
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1018
    Illegal SpeI site found at 1447
    Illegal PstI site found at 1461
    Illegal NotI site found at 1024
    Illegal NotI site found at 1454
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1018
    Illegal XhoI site found at 2478
    Illegal XhoI site found at 3504
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1018
    Illegal suffix found in sequence at 1447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1018
    Illegal XbaI site found at 1033
    Illegal SpeI site found at 1447
    Illegal PstI site found at 1461
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None