Plasmid

Part:BBa_K4182010

Designed by: YiBo Yuan   Group: iGEM22_XJTU-China   (2022-10-10)
Revision as of 16:19, 10 October 2022 by Registry (Talk | contribs)

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Critical synthesis circuitry from important precursor GPP to AA

Plasmid III uses the medium-copy plasmid MCS1 as the backbone (to avoid metabolic stress caused by high-copy plasmids), including the astABC trigene and a specific transcription terminator T1 from the E. coli rrnB gene regulated by the lac promoter, as well as multiple highly active ribosomal binding sites (RBS1-3). The astABC gene, LacI-Plac regulatory sequence, and MCS plasmid skeleton were obtained using PCR technology, respectively, and the final plasmid 3 was obtained by further one-step ligation using the golden gate technique .

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3626
    Illegal EcoRI site found at 7595
    Illegal EcoRI site found at 10513
    Illegal PstI site found at 5844
    Illegal PstI site found at 6024
    Illegal PstI site found at 7131
    Illegal PstI site found at 8462
    Illegal PstI site found at 10270
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3626
    Illegal EcoRI site found at 7595
    Illegal EcoRI site found at 10513
    Illegal PstI site found at 5844
    Illegal PstI site found at 6024
    Illegal PstI site found at 7131
    Illegal PstI site found at 8462
    Illegal PstI site found at 10270
    Illegal NotI site found at 2615
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3626
    Illegal EcoRI site found at 7595
    Illegal EcoRI site found at 10513
    Illegal BamHI site found at 5902
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3626
    Illegal EcoRI site found at 7595
    Illegal EcoRI site found at 10513
    Illegal PstI site found at 5844
    Illegal PstI site found at 6024
    Illegal PstI site found at 7131
    Illegal PstI site found at 8462
    Illegal PstI site found at 10270
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3626
    Illegal EcoRI site found at 7595
    Illegal EcoRI site found at 10513
    Illegal PstI site found at 5844
    Illegal PstI site found at 6024
    Illegal PstI site found at 7131
    Illegal PstI site found at 8462
    Illegal PstI site found at 10270
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 5610
    Illegal NgoMIV site found at 7733
    Illegal NgoMIV site found at 9063
    Illegal AgeI site found at 183
    Illegal AgeI site found at 7888
    Illegal AgeI site found at 9688
  • 1000
    COMPATIBLE WITH RFC[1000]


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