Composite

Part:BBa_K4509669

Designed by: Rhea Harry William, Vibha Murali, MARIE JYOTSNA JAWAHAR   Group: iGEM22_REC-CHENNAI   (2022-09-28)
Revision as of 10:04, 28 September 2022 by Registry (Talk | contribs)

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Cell surface aphA with Cd2+ sensing promoter

This part consists of a cadmium metal sensing promoter (BBa_K896008), strong RBS (BBa_B0034), cell surface tag (BBa_K103006) linked to acid phosphatase gene (BBa_K4509369), GFP reporter molecule with terminator (BBa_K259006). This part expresses acid phosphatase as a cell surface protein in the presence of cadmium. A GFP reporter molecule has been added to help measure its expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1012


[edit]
Categories
Parameters
None