Coding

Part:BBa_K4361103

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-06)
Revision as of 08:17, 11 October 2022 by Larsvdb (Talk | contribs)


BlcR with 6xHis-tag and TEV protease cleavage site

Composite part consisting of E. coli codon optimized BlcR (Part:BBa_K4361100, with a His-tag for purification and TEV cleavage site for separation of said tag from the protein (Part:BBa_K4361104). Through this combination, we were able to attain improved expression of our protein of interest and subsequently purify it without inhibiting its functionality.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 766
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 901
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 661


[edit]
Categories
//awards/composite_part
Parameters
None