Composite
Part:BBa_K4509769
Designed by: MARIE JYOTSNA JAWAHAR, Rhea Harry William, Vibha Murali Group: iGEM22_REC-CHENNAI (2022-09-28)
Cell surface aphA with constitutive promoter
This construct consists of strong promoter and strong RBS combination (BBa_K880005), cell surface tag (BBa_K103006) linked to aphA gene(BBa_K4509369) and GFP reporter attached to terminator (BBa_K259006). OmpA Linker tag helps to express acid phosphatase enzyme(aphA) on the cell surface. The reporter molecule attached will support in tracking the location of the protein and determining gene expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 966
[edit]
Categories
Parameters
None |