Composite

Part:BBa_K4140027

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-28)
Revision as of 04:51, 12 October 2022 by Mohamed-Ab0elghare (Talk | contribs) (→‎Part Description)


Permeability device

Part Description

In this composite, the Lac promoter regulates the expression of phenylalanine permease which is a single integral membrane transporter that uses the proton motive force to antiport L-phenylalanine and L-tyrosine. And present naturally in Escherichia coli.

Usage

We implement this composite to increase the permeability of cells to phenylalanine by the expression of phenylalanine permease that is a single, integral membrane transporter to improve the sensitivity of our circuit to phenylalanine as shown in figure 1 and figure 2.

Right

Figure 1. (shows the permeability circuit in our E.coli-based system.)

Right

Figure 2. (shows the whole cell-based biosensor.)

Experimental Characterization

Tube131.png








This figure shows an experimental characterization of this part as it's validated through gel electrophoresis as it is in lane 7. The run part (ordered from IDT) included Lac promoter - Permease.













Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 81
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None