Composite

Part:BBa_K4509469

Designed by: Vibha Murali, Rhea Harry William, MARIE JYOTSNA JAWAHAR   Group: iGEM22_REC-CHENNAI   (2022-09-28)
Revision as of 03:18, 12 October 2022 by Marie JJ (Talk | contribs)


csgD and OmpR234 Dual Expression

This composite part consists of a strong promoter(BBa_J23118),a strong RBS(BBa_B0034),transcriptional regulators of two curli operons - csgD (BBa_K805015) and OmpR234 (BBa_K342003) and a double terminator (BBa_B0015). The strong promoter and strong RBS are used for the overexpression of the desired proteins. By overexpressing both the proteins, curli fiber production in Escherichia coli K-12 can be increased.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 877
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

[edit]
Categories
Parameters
None